Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Lysophosphatidylglycerol inhibits formyl peptide receptor like-1-stimulated chemotactic migration and IL-1β production from human phagocytes

Figure 2

Effect of LPG on MMK-1-induced chemotactic migration in human monocytes. Isolated human monocytes (1 × 106 cells/ml of serum free RPMI 1640 medium) were added to the upper wells of a 96-well chemotaxis chamber. Various concentrations of MMK-1 or LPG were used for the chemotaxis (A, B). Chemotaxis assay was performed using MMK-1 in the presence of several concentrations (0, 1, 5, 10, and 20 µM) of LPG in the upper chamber (C). The migration across a 5 µm pore sized polycarbonate membrane was assessed after incubating at 37℃ for 2 h. The number of migrated cells was determined by counting the number of cells in 5 high power fields (400×). The data are expressed as the means ± SE from three independent experiments performed in duplicate (A-C). *P < 0.05 compared to the values obtained from the control (0 µM). #Significantly different from the control (MMK-1 only treated) (P < 0.05).

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