Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Interleukin-1β promotes the expression of monocyte chemoattractant protein-1 in human aorta smooth muscle cells via multiple signaling pathways

Figure 1

IL-1β induces MCP1 expression in HASMCs. (A) HASMCs were treated with 5 ng/ml IL-1β for the indicated times. Total RNA was isolated and RT-PCR analysis was performed using MCP1 gene-specific primers and the internal control gene, β-actin. Two additional experiments yielded similar results. A representative study is shown. (B) The amount of secreted MCP1 protein was determined in the supernatant after IL-1β treatment for the indicated times using the human MCP1 ELISA kit. Data are presented as mean values obtained from three independent experiments, and the bars represent standard deviations. The significance was determined by Student's t-test (*P < 0.05 vs untreated control).

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