Figure 1

Rosiglitazone and pioglitazone induce profound multilocularization in the adipocytes of adult WAT of mice. The indicated adipose tissues were harvested from C57BL/6J mice that had treated with nothing (Control), rosiglitazone (~15 mg/kg/day) (A, B) or pioglitazone (~15 mg/kg/day) (C, D) for 3 weeks. (A, C) Tissues were whole-mounted, co-immunostained for perilipin (for lipid droplets) and collagen IV (the basement membrane that surrounds each adipocyte), and merged. Note that adipocytes with variable and smaller-sized perilipin⁺ lipid droplets are abundantly detected in rosiglitazone- or pioglitazone-treated adipose tissues. Scale bars, 20 µm. (B, D) Multilocular adipocytes were counted in 10 regions (~100 adipocytes/region) per adipose tissue in each mouse of Control (n = 3), rosiglitazone (B, n = 4) or pioglitazone (D, n = 4), and presented as a percentage of the total adipocytes counted. Bars represent the mean ± SD. ^*, P < 0.01 versus control. ROSI, rosiglitazone; PIOG, pioglitazone.