Figure 3
Rosiglitazone induces less multilocularization in the adipocytes of adult subcutaneous adipose tissues of db/db and ob/ob obese mice than of normal mice. The indicated adipose tissues were harvested from C57BL/6J mice, db/db mice, and ob/ob mice that had treated with nothing (Control) or rosiglitazone (~15 mg/kg/day) for 3 weeks. (A) Tissues were whole-mounted, co-immunostained for perilipin (dark pink) and collagen IV (blue), and merged. Note that fewer small-sized adipocytes are detected in the subcutaneous adipose tissues of db/db mice and ob/ob mice compared those of normal mice. A higher magnification of the merged images (white dotted rectangles) reveals characteristic rosiglitazone-induced multilocular adipocytes. Scale bars, 50 µm. (B) Multilocular adipocytes were counted in 10 regions (~100 adipocytes/region) per subcutaneous adipose tissue in each mouse of Control (n = 3) or rosiglitazoneI (n = 4), and presented as a percentage of the total adipocytes counted. Bars represent the mean ± SD. *P < 0.01 versus control. #P < 0.05 versus Normal.
