Figure 1
Recoveries of CFU-F in the irradiated bone marrows. Mice were total body irradiated (900 rad) and bone marrow cells (1 × 105/mice) from transgenic mice expressing GFP were intravenously injected. Bone marrows (2 femurs and 2 tibias) of recipient mice were then harvested at various time points after irradiation and aliquots of the bone marrow cells were plated for 14 days to form CFU-F. The numbers of CFU-Fs formed in the plates were measured by crystal violet staining. Shown are the mean ± SEM of CFU-F numbers (A) and total mononuclear cell (MNC) numbers (B) contained in two femurs and tibia of mice at each time point of recovery (3 independent experiments). (C) Representative image of crystal violet staining for CFU-F obtained after plating 1 × 107 bone marrow cells from non-radiated and irradiated (24 h before) mice. (D) Lack of stromal contribution from intravenously transplanted bone marrows cells. CFU-Fs generated in (A) were trypsinized and passage cultured to enrich non-hematopoietic (CD45-negative) stromal cells and examined for stromal cells of donor origin (CD45-neg, GFP +). Shown are the representative flowcytometry profiles for subculture of CFU-Fs obtained from non-transplanted mice (control) and mice transplanted with GFP-transgenic mice bone marrow cells (transplanted).
