Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Intramarrow injection of β-catenin-activated, but not naïve mesenchymal stromal cells stimulates self-renewal of hematopoietic stem cells in bone marrow

Figure 3

Effects of naïve MSCs on in vivo self-renewal of co-transplanted HSCs. Lethally irradiated mice (Ly5.1) were intravenously transplanted with congeneic (Ly5.2) donor bone marrow cells (BMCs) along with intrafemoral (IF) injection of MSCs or PBS buffer. Sixteen weeks after transplantation, the number of regenerated CRUs in the recipient mice was determined by limiting dilution transplantations of primary recipient mice marrow into secondary recipients. (A) Schematic illustration of the experimental design is shown. (B) The results of the CRU assays are shown. CRU frequencies and 95% confidence interval (C.I.) of the frequencies were obtained by applying Poisson statistics to the percent of negatively engrafted mice at different cell doses. The number of CRUs regenerated in mice was calculated based on the assumption that the cells in two femurs and tibias represent 25% of the total bone marrow as described (Boggs, 1984).

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