Figure 4

Effect of ethanol on AMPK expression. Tissues were prepared the same as in Figure 2. AMPKα1 (A) and α2 (B) mRNA, total- and phosphorylated-AMPKα protein (C) levels were measured by RT-PCR and western blotting, respectively. The quantification processes were similar with those in Figure 3. ^**P < 0.01, ^*P < 0.05 compared with control group.