Figure 10 | Experimental & Molecular Medicine

Figure 10

From: Activation of AMP-activated protein kinase stimulates the nuclear localization of glyceraldehyde 3-phosphate dehydrogenase in human diploid fibroblasts

Figure 10

SFM- and AICAR-induced GAPDH translocation in p53-null cells. Normal and p53-null MEF cells (A and B) and HCT116 cells (C and D) were cultured in 10% FBS medium and then treated with SFM for the times indicated (A and C) or with various concentrations of AICAR in 10% FBS for 2 days (B and D). Cells were immunostained against GAPDH and analyzed by confocal laser scanning microscopy, as described in Figure 1. The number of cells with cytosolic GAPDH alone (cytosol) and cells having nuclear GAPDH with or without cytosolic GAPDH (nuclear +/- cytosol) was counted, and the percent distributions are plotted as means ± standard deviations. *P < 0.05 and ***P < 0.001, comparison between normal cells and p53-null cells.

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