Figure 11

Effect of the CRM1 inhibitor LMB on the GAPDH distribution. HDFs were cultured in 10% FBS medium for 2 days and treated with vehicle (methanol) or various concentrations of LMB (1-20 ng/ml) for 2 days (A) or with 10 ng/ml LMB for the times indicated (B). In another experiment (C), HDFs were cultured in 10% FBS medium and serum-depleted by incubation with SFM for 4 days (SFM-4d). FBS was added to serum-depleted cells in the absence (0) or presence of various concentrations of LMB for 2 days. Cells were immunostained against GAPDH and analyzed by confocal laser scanning microscopy, as described in Figure 1. The number of cells with cytosolic GAPDH alone (cytosol) and cells having nuclear GAPDH with or without cytosolic GAPDH (nuclear +/- cytosol) was counted, and the percent distributions are plotted as means ± standard deviations. ^***P < 0.001, comparison between the FBS control (0) and FBS + LMB (ng/ml).