Figure 9 | Experimental & Molecular Medicine

Figure 9

From: Activation of AMP-activated protein kinase stimulates the nuclear localization of glyceraldehyde 3-phosphate dehydrogenase in human diploid fibroblasts

Figure 9

Effect of NOS inhibitors on SFM- and AICAR-induced GAPDH translocation. HDFs were cultured in 10% FBS medium, and then treated with SFM for 4 days or 2 mM AICAR in 10% FBS in the absence (-) or presence (+) of 100 µM of L-NMMA (A) or 0.5 mg/ml L-NAME (B) for 2 days. Cells were immunostained against GAPDH and analyzed by confocal laser scanning microscopy as described in Figure 1. The number of cells with cytosolic GAPDH alone (cytosol) and cells having nuclear GAPDH with or without cytosolic GAPDH (nuclear +/- cytosol) was counted, and the percent distributions are plotted as means ± standard deviations. *P < 0.05, comparison between the absence (-) and presence (+) of NOS inhibitors.

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