Figure 5 | Experimental & Molecular Medicine

Figure 5

From: Co-treatment with hepatocyte growth factor and TGF-β1 enhances migration of HaCaT cells through NADPH oxidase-dependent ROS generation

Figure 5

PI3K pathway was involved in cell migration, but not in ROS generation. (A) and (B) HaCaT cell migration was inhibited by PI3K inhibitors. HaCaT cells were pretreated for 1 h with wortmannin (A) or LY294002 (B) at the indicated concentrations before scratching. Growth factors were added as indicated just after the scratch. Data are presented as mean ± SD of two independent experiments. (**; P < 0.01 compared to that without inhibitors by student's t-test). (C) and (D) Wortmannin (1 µM) and LY294002 (30 µM) could not abolish the increase of ROS. HaCaT cells were prepared as indicated (A). Then, cells were incubated for 10 min with DCF-DA harvested for FACS analysis. Peak fluorescence intensity was measured for each sample, and a representative data from two independent experiments are shown. Vertical dashed line was drawn to help compare the location of two peaks.

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