Figure 5
Characterization of transglutaminase 4 isoforms expressed in yeast and HeLa cell lysate. (A) Yeast INVSc1 cells were transformed with pYes2.0 vector containing cDNA for hemagglutinin-tagged transglutaminase 4 isoform. Yeast lysates were analyzed by Western blot analysis using anti-hemagglutinin antibody. (B) transglutaminase activities of yeast lysates were estimated by measuring incorporation of [14C]-putrescine to N,N'-dimethylcasein. Fold increase of transglutaminase activity is expressed as a relative value to that of vector transfected cells, which was normalized by protein level of transglutaminase 4 isoforms. (C) HeLa cells were transfected with cDNA for hemagglutinin-tagged transglutaminase 4 isoforms. Cell lysates and lyophilized culture supernatants were analyzed by Western blot analysis using anti-hemagglutinin antibody. L, transglutaminase 4-L; M1, transglutaminase 4-M1; M2, transglutaminase 4-M2.
