Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Involvement of MITF-A, an alternative isoform of mi transcription factor, on the expression of tryptase gene in human mast cells

Figure 2

The transcriptional activity of beta tryptase gene in human mast cell line HMC-1. (A) The nucleotide sequence of 5' flanking region of the tryptase gene. The CANNTG motif was boxed. A part of the first exon is shown by capitals, and the 5' flanking region is shown by lower case. The transcription initiation site was numbered as + 1. (B) The reporter plasmids (0.5 µg) were transfected into HMC-1 by electroporation, but they (1 µg) were into Jurkat, and 293 cells by transfection reagent. Luciferase activity assay was performed as described in the Methods. Data revealed as the relative luciferase activity divided by a value from the empty plasmid.

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