Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Involvement of MITF-A, an alternative isoform of mi transcription factor, on the expression of tryptase gene in human mast cells

Figure 3

The transcriptional activity of deletion mutants in 5' flanking region of beta tryptase gene in the HMC-1 and Jurkat cells. (A) The tryptase promoter region (-817~ + 24) was obtained from the genomic DNA of HMC-1 by PCR. The deleted constructs of beta-tryptase promoter were synthesized and inserted into upstream of luciferase gene in pGL3-basic plasmid as reporter for transcriptional activity assay. (B) The reporter plasmids (0.5 µg) were transfected into HMC-1 cells. (C) The reporter plasmids (0.5 µg) were cotransfected to Jurkat cells with or without MITF expression vector (0.5 µg). The luciferase activity assay was performed as described in the Methods. Data revealed as the relative luciferase activity divided by the value of the empty reporter (pGL3- basic).

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