Figure 7

The decreased expression of tryptase by MITF depletion in HMC-1 cells. After transfection of human MITF-A specific siRNA (1 or 0.2 µg) or control siRNA (1 µg) into HMC-1 cells, (A) the depletion of MITF were analyzed by western blot analysis, and (B) the expression of tryptase mRNA and (C) tryptase enzymatic activity were measured as described in the Methods.