Figure 4

Up-regulation of Egr-1 expression by CPZ. (A, B) Western blot analysis. Serum-starved (0.5% serum for 24 h) C6 cells were treated with different concentrations of CPZ for 1 h (A) or 20 µM CPZ for different times (B). Cellular extracts were analyzed using Western blotting using the anti-Egr-1 antibody. The same blot was re-probed with anti-ERK2 antibody as an internal control. Each blot represents three individual experiments. (C) Northern blot analysis. Serum-starved (0.5% serum for 24 h) C6 cells were treated with 20 µM CPZ for the indicated time periods. Total RNA (10 µg) was isolated and Northern blot analysis was performed with the ³²P-labeled Egr-1 cDNA probe. gapdh mRNA was used as a control to verify the amount of RNA in each lane. Each blot represents at least three individual experiments.