Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Enhancement of antitumor effect using dendritic cells activated with natural killer cells in the presence of Toll-like receptor agonist

Figure 1

DC characteristics in maturation with NK cells. (A) Optimal ratio of DC versus NK cell. Immature DCs (iDCs) were co-cultured with NK cells at various ratios from 1:1 to 1:10. Culture supernatants were analyzed for IL-12p70 production. DCs maturated with NK cells at a ratio of 1:2 produced higher level of IL-12p70 than other ratios (*, P <0.05). The data from a representative of three independent experiments are shown. (B) Comparison of phenotype of mature DCs. iDCs were co-cultured with NK cells at ratio of 1:2 in the presence of LPS and/or IL-2. The expressions of I-Ad, CD40, CD80 and CD86 were analyzed by flow cytometry. DCNK+LPS+IL-2 were expressed the highest levels of these molecules. Shown are representative histograms demonstrating the expression of markers (shaded) compared to isotype controls (black line). (C) Bar graphs showed the mean % expression (+SD) from five independent experiments. *, P < 0.05 compared to DCLPS. There were no significant effects of tumor antigens (TA) on the expressions of surface molecules on DCs.

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