Figure 3

The allostimulatory capacity of DCs. Splenocytes (A) or CD90+ T cells (B) (50,000 cells/well) obtained from allogeneic mice were stimulated with graded doses of irradiated DCs for 5 days, and [3H]-methylthymidine was added 18 h before measurement of proliferative response. The stimulatory capacity of DCNK+LPS+IL-2 was significantly higher in splenocytes and T cells than other DCs (*, P < 0.05). Data shown are the mean cpm (± S.D) of triplicate cultures from three independent experiments.