Figure 4
Tumor antigen uptake of DCs and tumor specific IFN-γ secreting CTLs. (A) DCs were loaded with PKH26-labeled UVB-irradiated CT26 cells at a ratio of 2:1. CD11c+ mature-DCs, identified by H-2k or CD80 expression, were analyzed for the uptake of PKH26 labeled tumor cells. DCNK+LPS and DCNK+LPS+IL-2 showed more efficiently take up irradiated tumor cells compared to DCLPS. The data from a single representative experiment are shown. (B) Autologous T cells were stimulated with irradiated-pulsed or unpulsed DCs. The CTLs were co-cultured with target cells (CT26 cells and CT26-pulsed-DCs), and were subjected to the IFN-γ release ELISPOT assay. The CTLs stimulated with the pulsed-DCNK+LPS+IL-2 demonstrated significantly higher IFN-γ-secreting cells against target cells than other CTLs (*, P < 0.05). A few IFN-γ secreting cells were produced when YAC-1 was used as target cells. The data are shown as the mean numbers of IFN-γ-secreting cells (± S.D) of triplicate culture from a representative data from three independent experiments.
