Figure 3
From: Msx2 mediates the inhibitory action of TNF-α on osteoblast differentiation
TNF-α-inhibited ALP expression is rescued by the knockdown of Msx2. To induce the silencing of Msx2, C2C12 cells were transfected with siRNAs for Msx2 (si Msx2) or control siRNAs (si control) and further incubated in the presence of the indicated reagents for 24 h. Next, RT-PCR, immunoblot analysis and ALP staining were performed. Lamin B was used as a loading control.
