Figure 5
From: Msx2 mediates the inhibitory action of TNF-α on osteoblast differentiation
JNK activation is not involved in TNF-α-induced Msx2 expression. (A, C) C2C12 cells were treated with the indicated reagents for 5 min (A) or for 24 h (C) and then immunoblot analysis and RT-PCR were performed. JNK activation was confirmed using whole cell lysates whereas the Msx2 protein level was examined using nuclear extracts. (B) SP600125 did not exert any cytotoxic effects at a concentration of 10 µM. Data represent the mean + SD of quadruplicates. *, P < 0.01 (Student's t-test)
