Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Synovial fluid of patients with rheumatoid arthritis induces α-smooth muscle actin in human adipose tissue-derived mesenchymal stem cells through a TGF-β1-dependent mechanism

Figure 1

Effects of RA-SF on the expression of α-SMA in hASCs. (A) Serum-starved hASCs were treated with indicated concentrations of SF with RA patients or normal patients for 4 days. (B) Serum-starved hASCs were treated with vehicle, 1% RA-SF or 0.2 ng/ml TGF-β1 for the defined times. (C) Serum-starved hASCs were treated with vehicle (w/o), 1% SF of five normal donors, or 1% SF of six RA patients for 4 days. (D) The densities of α-SMA were quantified from three independent experiments, and the expression levels of α-SMA were normalized to total GAPDH levels in the samples. The data are presented as a percentage of control. *, P < 0.05.

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