Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Synovial fluid of patients with rheumatoid arthritis induces α-smooth muscle actin in human adipose tissue-derived mesenchymal stem cells through a TGF-β1-dependent mechanism

Figure 2

Effects of RA-SF on the formation of actin stress fibers and the localization of α-SMA in hASCs. Serum-starved hASCs were treated with vehicle, 1% RA-SF, or 0.2 ng/ml TGF-β1 for 4 days, and immunofluorescence staining was performed. α-SMA was stained with anti-α-SMA antibody and probed with Alexa Fluor 488-conjugated anti-mouse secondary antibody. F-actin was detected with Alexa Fluor 568 phalloidin and the double stained images were analyzed by confocal microscope (400× magnification). The merged images of α-SMA (green) and F-actin (red) are shown. Representatives of three independent experiments are shown.

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