Figure 6 | Experimental & Molecular Medicine

Figure 6

From: Synovial fluid of patients with rheumatoid arthritis induces α-smooth muscle actin in human adipose tissue-derived mesenchymal stem cells through a TGF-β1-dependent mechanism

Figure 6

Role of Smad2 in the RA-SF-induced expression of α-SMA. (A) Serum-starved hASCs were transfected with control si-RNA (Control) or Smad2 si-RNA (si-Smad2), and then exposed with 1% RA-SF or 0.2 ng/ml TGF-β1 for 4 days. (B) hASCs were infected with adenoviruses carrying β-galactosidase (Ad-LacZ) or FLAG-tagged Smad7 (Ad-Smad7), and then treat with vehicles or 1% RA-SF and 0.2 ng/ml TGF-β1 for 4 days. The expression levels of α-SMA, Smad2, FLAG-tagged Smad7, and GAPDH were determined by Western blotting with anti-α-SMA, anti-Smad2, anti-FLAG and anti-GAPDH antibodies. Phosphorylation of Smad2 was determined by Western blotting with anti-p-Smad2. Representatives of three independent experiments are shown.

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