Figure 1
From: Transglutaminase 2 inhibits apoptosis induced by calciumoverload through down-regulation of Bax
TG2 inhibits A23187-induced cell death. (A) TG activity in HEK293 cells overexpressing TG2 (293TG2-2 and 293TG2-5). TG activity was expressed as relative value using HEK293 cells as a control. The level of TG2 expression was estimated by Western blot analysis (inset). (B) A23187-induced cell death in HEK293, 293TG2-2 and 293TG2-5 cells. Cell death was quantitated by trypan blue staining after 48 h of treatment with 5 µM A23187. (C) Time-dependent and dose-dependent cytotoxicity of A23187 in HEK293 and 293TG2-5 cells. (D) Flow cytometry analysis of A23187-induced apoptosis in HEK293 and 293TG2-5 cells determined by propidium iodide staining after treatment with 5 µM A23187. M1 represents sub-G1 population. Data represent the mean ± SD from three independent experiments. Asterisks indicate statistically significant differences (*, P < 0.05; **, P < 0.01) compared with HEK293 cells.
