Figure 4

Quantitative RT-PCR. RNA was isolated from the hearts of mice from the four experimental groups and subjected to quantitative RT-PCR. The expression levels of sarcoplasmic reticulum Ca²⁺ ATPase (SERCA2a) and alpha-myosin heavy chain (α-MHC) were significantly reduced following transverse aortic banding (TAB), whereas the levels of brain natriuretic peptide (BNP), TGF-β1, and collagen 1 were significantly increased. Note that these changes in the gene expression were more profound with parathyroid hormone (PTH) treatment. The numbers of mice available for this analysis were as follows: Sham/Saline (n = 3), Sham/PTH (n = 3), TAB/Saline (n = 4), and TAB/PTH (n = 4). Data are presented as mean ± SD. ^*P < 0.05, ^**P < 0.01.