Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Estrogen modulates transactivations of SXR-mediated liver X receptor response element and CAR-mediated phenobarbital response element in HepG2 cells

Figure 1

Effects of estrogen, rifampicin, and corticosterone on the synthetic (4ERE)-tk-luciferase transactivation in HepG2 cells. HepG2 cells were transfected with 250 ng of 4ERE-tk-luciferase reporter vector and 10 ng of pRLSV40 as an internal control for transfection efficiency in the presence of pCMX-RXR (1 ng), pCMV-hERĪ± (5 ng), and pCMX-SXR (5 ng). The ligands were added for 24 h after transfection as indicated: 10 nM of 17Ī²-estradiol (E2) for ER and 10 ĀµM of rifampicin (Rif) or 10 ĀµM of corticosterone (Ct) for SXR. The cells were harvested for dual luciferase assays. The values for firefly luciferase were normalized by dividing by Renilla luciferase values. The standard errors of the mean were calculated from four independent transfection experiments. Bars with different superscript letters differ significantly (P < 0.05).

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