Figure 1

Aurora-A overexpression elevated c-Myc expression and transcriptional activity. (A) The establishment of Aurora-A-inducible cell line. Cell lysates from 293-TR-Aur-myc or 293-TR-Vec treated with or without Dox (2 µg/ml) for 24 h were subjected to Western blotting with anti-myc antibodies. (B) The mRNA and protein level of c-Myc upon Aurora-A induction. 293-TR-Aur-myc cells were treated with or without Dox for 24 h and then harvested for RT-PCR and Western blotting. β-actin was used as a loading control. (C) The transcriptional activity of c-Myc upon Aurora-A induction. The 293-TR-Aur-myc cells were transfected with the indicated plasmids and luciferase activity was measured 48 h after transfection. The pcDNA3.0-c-Myc was used as a positive control for the constructed E-box reporter system. Transfection efficiency was normalized by co-transfection of pRL-TK. All experiments were performed three times.