Figure 2

c-Myc RNAi attenuated the increased cellular proliferation rate conferred by Aurora-A overexpression. (A) The efficiency of c-Myc RNAi plasmid. The 293-TR-Aur-myc cells were transfected with pSilencer-c-Myc or control plasmid for 48 h and then treated with Dox for 24 h. Cells lysates were subjected to Western blotting for detection of c-Myc expression. (B) Cell growth curves. The 293-TR-Aur-myc cells (5 × 10⁴) were transfected with pSilencer-c-Myc or control plasmid and then treated with or without Dox. Cell numbers were counted every 24 h. The data are shown as mean ± SD for three independent cultures. (C) The proliferation rates were measured using a BrdU assay. The 293-TR-Aur-myc cells were treated with Dox after transfection with pSilencer-c-Myc or control plasmid. Then BrdU incorporation was performed to measure the proliferation rates, ^**P < 0.001.