Figure 4

c-Myc RNAi alleviated centrosome abnormalities induced by Aurora-A overexpression. (A) The abnormal centrosomes induced by Aurora-A. The 293-TR-Aur-myc cells were incubated with Dox for 48 h and then triple-stained with DAPI (blue), anti-α-tubulin (green) and anti-γ-tubulin (red) to show the excessive numbers of centrosomes. (B) The percentage of cells with centrosome abnormalities. The 293-TR-Aur-myc and 293-TR-Vec cells were treated with Dox for the indicated time intervals. The cells with abnormal centrosomes were counted (at least 200 cells were counted). (C) The percentage of cells with centrosome abnormalities in 293-TR-Aur-myc cells with prior transfection of pSilencer-c-Myc or control plasmid. At least 200 cells were counted.