Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Enhancement of parthenolide-induced apoptosis by a PKC-alpha inhibition through heme oxygenase-1 blockage in cholangiocarcinoma cells

Figure 2

Apoptotic cell death and Nrf2 and HO-1 expression in CC cells treated with various inhibitors. (A) Apoptotic cell death in Choi-CK and SCK cells treated with the indicated inhibitors for 72 h and then stained with Hoechst 33258. The fraction of apoptotic cells was determined using fluorescence microscopy. Each data point represents the mean ± SE of three independent experiments performed in duplicate. *, P < 0.05; **, P < 0.01. (B) Nrf2 expression in Choi-CK cells treated for 72 h with various inhibitors at the indicated concentration. (C) HO-1 expression in Choi-CK cells treated with various inhibitors at the indicated concentrations for 72 h. (D) Expression of Nrf2 and HO-1 in SCK cells treated with Ro at the indicated concentrations for 72 h. For each blot, initial immunodetection was performed, and then the blots were stripped and reprobed with a monoclonal antibody to actin as a loading control. All immunoblot experiments were performed at least three times, and the result of one representative experiment is shown.

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