Figure 5 | Experimental & Molecular Medicine

Figure 5

From: Enhancement of parthenolide-induced apoptosis by a PKC-alpha inhibition through heme oxygenase-1 blockage in cholangiocarcinoma cells

Figure 5

HO-1 suppression enhanced apoptotic cell death in SCK cells. (A) SCK cells were transfected with 50 nM siRNA targeting HO-1 (HMOX1-1 and HMOX1-5) or a non-targeting siRNA, and the efficiency of HO-1 silencing was determined by immunoblot analysis using actin as a loading control (upper panels). (B) Cells were transfected with 50 nM HO-1 siRNA for 24 h and subsequently treated with either 10 µM or 20 µM PTL for 72 h prior to staining with Hoechst 33258. The fraction of apoptotic cells was determined using fluorescence microscopy. Each value represents the mean ± SE of three independent experiments. *, P < 0.01.

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