Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Cyclic AMP prolongs graft survival by suppressing apoptosis and inflammatory gene expression in acute cardiac allograft rejection

Figure 2

Treatment with db-cAMP inhibits the expression of adhesion molecules in the transplanted hearts of allograft recipient rats. Transplanted hearts were harvested on POD 5 and total mRNAs were isolated using a Trizol reagent kit. (A) Typical expression patterns of ICAM-1 and VCAM-1 in transplanted hearts were obtained by RT-PCR. (B) The expression levels of ICAM-1 and VCAM-1 were determined by qrPCR analysis, and the mRNA levels were calculated from the mRNA ratio of adhesion molecule gene/GAPDH. Data shown are the mean ± SD (n = 4). *P < 0.05 versus non-treated allograft.

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