Figure 4
From: Up-regulation of BLT2 is critical for the survival of bladder cancer cells
Effects of blockade of the BLT2-NOX signal on the ERK/AKT pathway in 253J-BV cells. (A) Relative expression of ERK and AKT in the human bladder cancer cell lines 253J and 253J-BV compared with the control SV-HUC-1. (B) Knockdown of BLT2 by specific siRNA reduced phosphorylation of ERK and AKT in 253J-BV cells. Cells were transfected with BLT2 siRNA (50 nM) for 36 h and proteins were separated by SDS-PAGE (8%), transferred onto PVDF membranes, and detected with specific antibodies. (C) Knockdown of NOX1/4 reduced phosphorylation of ERK and AKT in 253J-BV cells. pSUPER and pSUPER-siNOX1/4 (1 µg) were transiently transfected into 253J-BV cells. After 36 h, the cells were harvested for detection of ERK and AKT with specific antibodies. Results are representative of three independent experiments with similar results.
