Figure 2
N-BPs enhance TRAIL-induced apoptosis of MG 63 cells. (A) MG 63 cells were co-treated with 50 µM alendronate for 24 h with/without 400 ng/ml TRAIL for an additional 12 h. Following treatment, cells were fixed and immunostained with anti-BrdU antibody to observe the extent of DNA damage and counterstained with propidium iodide. The level of staining indicated the degree of DNA damage induced by treatment, where more positively stained cells were in the final stages of apoptosis. The magnification is ×200. (B-E) MG 63 cells were pretreated with 50 µM alendronate (B) or 100 µM risedronate (D) 10 µM GGOH and/or cholesterol and/or 100 µM Z-VAD-fmk (E) for 24 h with/without 400 ng/ml of TRAIL for an additional 4 h. Cell lysates were analyzed by immunoblotting using antibodies for cleaved caspase-3. (C) MG 63 cells were treated as described in Figure 2A and the cells were fixed and stained with cleaved caspase-3 and Alexa flour 546-conjugated secondary antibody, followed by fluorescence microscope analysis.
