Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Hydroxydibenzoylmethane induces apoptosis through repressing ornithine decarboxylase in human promyelocytic leukemia HL-60 cells

Figure 3

Overexpression of ODC prevented HDB-induced apoptosis. HL-60 cells were transfected with pcDNA3 and ODC-pcDNA3 plasmids, and then cells were harvested to measure ODC protein (A) and enzyme activity (B). HL-60, pcDNA3 and ODC-pcDNA3 cells were treated with HDB for 12 h. The cells were harvested to measure morphology (C), the percentage of sub-G1 (D) and DNA fragmentation (E) by light microscope, flow cytometry using PI staining and DNA gel electrophoresis. (F) Prior to HDB treatment, ODC-pcDNA3 cells were pretreated with vehicle (control) or 1 mM DFMO for 1 h, or transfected with Luc shRNA or ODC shRNA for 12 h. Genomic DNA was collected and analyzed for DNA fragmentation. Data were representative of at least three experiments. *P < 0.05 as compared with H6-60 cells.

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