Figure 5
NAC, a ROS scavenger, decreased intracelluar ROS generation, the loss of Δψm and sub-G1 peak ratio after HDB treatment. (A) Parental HL-60 cells were pretreated with 10 mM NAC for 1 h and treated with or without HDB for 12 h. Intracellular ROS was detected by flow cytometry measuring fluorescence of DCF. Δψm was detected by flow cytometry with measuring fluorescence of rhodamine 123. (B) The percentage of sub-G1 was measured by flow cytometry using PI staining. Data were representative of at least three experiments.
