Figure 7
Overexpression of ODC protected cytochrome c release and the cleavage of caspase-3. pcDNA3 and ODC-pcDNA3 cells were treated with HDB for the indicated time. Cytosol and mitochondrial proteins were separated and detected by immunoblotting with anti-cytochrome c, heat shock protein 60 (HSP60, as a mitochondrial marker) and Glyceraldehyde-3-phosphate dehydrogenase (GADPH, as a cytosolic marker) antibodies. The all of the proteins were extracted for immunoblotting with specific antibody of caspase-3. *Indicated the activated and cleaved forms of caspase-3. Data were representative of at least three experiments.
