Figure 1

LFE inhibits adipocyte differentiation. (A) Mouse 3T3-L1 preadipocytes were differentiated into adipocytes in the presence of various concentrations of LFE for 6 days. Intracellular lipids were stained with Nile Red and fluorescence intensity was measured. Relative lipid accumulation was calculated as follows: (fluorescence intensity of treated cells / fluorescence intensity of DMSO-treated control cells) ×100. (B) After staining with Nile Red, cells were counter-stained with Hoechst 33342 and photographed with IN Cell Analyzer 1000 (GE Healthcare). (C) Cells were harvested at day 6 and the expression of PPARγ and C/EBPα was analyzed by Western blotting. (D) Total RNA was extracted at day 6 and the expression level of PPARγ, FAS, ACC1, SCD1, aP2, and adiponectin mRNA was analyzed by qPCR. Each bar represents mean ± SD of triplicate PCR reactions. Similar results were obtained from two independent experiments. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001.