Figure 2

LFE suppresses the expression and activation of PPARγ. (A) Differentiated 3T3-L1 adipocytes were treated with DMSO or LFE (2 or 10 µg/ml) for 24 h. Total protein was extracted and the expression of PPARγ and C/EBPα was analyzed by Western blotting. Experiment was done in duplicate. (B) HEK 293 cells were transfected with DR-1-luciferase reporter along with PPARγ and RXRα expression vectors. After 6 h, cells were treated with DMSO, rosiglitazone (1 µM), BADGE (100 µM), or LFE (10 µg/ml) and incubated for 24 h. Experiments were done in quadruple and luciferase activity was measured and expressed as mean ± SD. Similar results were obtained from two independent experiments. ^*P < 0.05 vs. lane 2, ^#P < 0.05 vs. lane 3.