Figure 3

LFE modulates the expression of genes involved in lipid metabolism. Differentiated 3T3-L1 adipocytes (A) or C2C12 cells (B) were treated with 10 µg/ml LFE for 24 h. Total RNA was isolated and analyzed by qPCR for the expression of lipogenic genes (ADD1/SREBP1c, FAS, ACC1 and SCD1) or lipolytic genes (ACO1, CPT1, PPARα and PPARδ). Each bar represents mean ± SD of triplicate PCR reactions. Similar results were obtained in two independent experiments. *P < 0.05, **P < 0.01.