Figure 5

LFE ameliorates obesity induced by HFD. Mice were fed either a ND or HFD for 6 weeks in the presence (30, 100, and 300 mg/kg/day) or absence of LFE (n = 8). (A) Changes in body weight. (B) Absolute amount of food intake per mouse. (C) Comparison of fat pad weight from abdominal subcutaneous, epididymal, and perirenal WATs. (D) Histological analysis of the epididymal WATs after staining with hematoxylin and eosin. (E) Effect of LFE on the expression of genes involved in lipid metabolism in the epididymal WAT. (F) Effect of LFE on the expression of C/EBPα and PPARγ proteins in the epididymal WAT. Epididymal WATs were obtained from two animals of each group and each lane represents the protein level of individual WAT. (G) Effect of LFE on the phosphorylation of AMPK in the epididymal WAT. Each bar represents mean ± SD from eight mice. ^*P < 0.05 vs. ND+Vehicle, ^***P <0.001 vs. ND + Vehicle, ^#P < 0.05 vs. HFD + Vehicle, ^##P < 0.01 vs. HFD + Vehicle, ^###P < 0.001 vs. HFD + Vehicle.