Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Benzoxathiol derivative BOT-4-one suppresses L540 lymphoma cell survival and proliferation via inhibition of JAK3/STAT3 signaling

Figure 1

BOT-4-one inhibits phosphorylation and transcription of STAT92E in Drosophila cells. (A) The chemical structure of BOT-4-one (C14H19NO2S; M.W., 265.4). (B) BOT-4-one inhibited cytokine (upd)-induced tyrosine phosphorylation of STAT92E. S2-NP cells transiently transfected with an expression plasmid for STAT92E-HA were co-cultured with upd-producing cells for 24 h in the presence of either vehicle (DMSO) alone or BOT-4-one. Immunoblot analysis was performed with phospho-STAT92E and HA antibodies. STAT92E-HA served as a loading control. (C) BOT-4-one inhibited STAT92E transcriptional activity. Cultured Drosophila S2-NP-STAT92E cells expressing a STAT92E reporter gene were co-cultured with upd-producing cells for 24 h in the presence of BOT-4-one. The STAT92E luciferase activity was measured and the firefly luciferase activity was normalized to Renilla luciferase activity. Results are shown as the mean of three independent experiments (± SD indicated by error bar). *P < 0.001, significant difference when the value of treatment was compared to that of the control.

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