Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Benzoxathiol derivative BOT-4-one suppresses L540 lymphoma cell survival and proliferation via inhibition of JAK3/STAT3 signaling

Figure 3

BOT-4-one inhibits JAK/STAT3 signaling. L540 (A), MDA-MB-468 (B), and DU145 (C) cells were incubated with either vehicle (DMSO) alone or BOT-4-one for 24 h. Whole cell extracts were proceeded for immunoblot analysis using antibodies specific for the molecules indicated. BOT-4-one inhibited constitutively-active STAT3 in all cell lines. Interestingly, BOT-4-one predominantly inhibited JAK3-mediated STAT3 phosphorylation and SOCS3 expression known to STAT3 downstream target molecule than those of JAK2-mediated. However, BOT-4-one inhibited ERK1/2 phosphorylation in a cell type-dependent manner, and has weak effect on Src family kinases such as Src and Lyn in all cell lines. GAPDH served as a loading control.

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