Figure 3
MIP2 involves in the HPoC-induced protection against H/Re mediated LDH release in H9c2 myogenic cells. Bar graphs showed the quantitative mRNA expression (A) and protein levels (B) of MIP2 in the H9c2 myogenic cells at 1, 3, 6, 9, 12, 15 and 24h after the reoxygenation (n = 3). Values are expressed as ratio versus GAPDH, *P < 0.05 vs sham group; #P < 0.05 vs H/Re group. Values are means ± SEM. Transfection with pcDNA3.1-MIP2 and MIP2 siRNA influenced MIP2 protein expression in H9c2 cells (C), MIP2 overexpression and suppression affected HPoC induced protective effect as indicated by LDH release into the medium (D). *P < 0.05 vs the pcDNA3.1 group. #P < 0.05 vs the control siRNA. Values are means ± SEM.
