Figure 5

Increased expression of IDO, TLR3 and TLR4 in synovium of patients with rheumatoid arthritis. (A) IDO, TLR3 and TLR4 expression (brown) was detected by immunohistochemistry in synovium of RA patients with severe or mild disease and OA patients. Nuclei were stained by hematoxylin (blue). An isotype control antibody served as negative control (Original magnification × 400). (B) The endogenous expression of IDO in synovial fibroblasts of RA and OA patients was examined by real time RT-PCR. Data are presented as mean ± SD of five independent experiments. *P < 0.05, severe RA vs. mild RA or OA.