Figure 5

Reduced expression of phospho-JNK levels in developing brains of jsap1^-/-. (A-J) Anti-phospho-JNK stained coronal sections of cryo-sectioned brains at E14.5 (A, B), E16.5 (C, D), and E17.5 (E-J). Phospho-JNK was distributed in the soma and neuritic processes of neuronal cells in broad regions of developing jsap1^+/+ and jsap1^-/- brains. Anti-phospho-JNK labeled neuronal cells in the marginal zone, subplate, intermediate zone, subventricular zone, caudoputamen, and cingulum bundles, and also labeled radial glial fibers in the cortical plate (G, H). G and H are magnified images of the rectangles in the insets. The expression level of phospho-JNK in the U-shaped axon tract of the corpus collasum (C-F), and anterior commissure (I, J) in jsap1^-/- brains was lower than that in jsap1^+/+ brains. (K) Quantification of the expression levels of phospho-JNK in indicated brain regions in both genotypes. ^*, P < 0.05 and ^**, P < 0.01, differences between jsap1^+/+ and jsap1^-/- brains (Student's t-test). AC, anterior commissure; CC, corpus callosum; cng, cingulum bundle; CP, caudo-putamen; CxP, cortical plate; ic, internal capsule; IZ, intermediate zone; LV, lateral ventricle; MZ, molecular zone; Pb, Probst's bundle; SP, subplate; tca, thalamocortical axons; VZ, ventricular zone. A, C, E, and G; jsap1^+/+. B, D, F, and H; jsap1^-/-. Scale bars: A-F, 250 µm; G and H, 100 µm.