Figure 2
Protective effect of continuous nonlethal hypoxia on PQ-induced cytotoxicity in A549 cells. (A) MTT Assay: Degree of viability was quantified at 24 h after 1 mM PQ treatment using the MTT reduction assay. PQ + Normoxia, 24-h normoxia (21% O2) after 1 mM PQ treatment for 8 h; PQ + Hypoxia, 24-h hypoxia (1% O2) after 1 mM PQ treatment for 8 h (B) LDH Assay: Inhibition of PQ (0.5 and 1 mM)-induced LDH release by hypoxia. Cell death was assessed by measuring the release of the cytosolic marker LDH and is expressed as percentage of net LDH release induced by PQ (% of total LDH, n = 6) Results are expressed as a percentage of the value obtained in normoxic cultures (control, not subjected to PQ treatment). #Significantly different from control group, P < 0.05. ##Significantly different from PQ + Normoxia group, P < 0.05.
