Figure 6
From: Down-regulation of survivin suppresses uro-plasminogen activator through transcription factor JunB
Effects of survivin on stable JunB-shRNA cells. Control cells and stable JunB-shRNA cells (1 × 106/well) were plated overnight in complete medium, starved for 24 h, treated with or without 10 ng/ml HGF for 1 h and harvested. The levels of expression of survivin were analyzed by Western blotting (A). To demonstrate HGF and JunB induced survivin promoter activity, stable JunB-shRNA cells and control cells were co-transfected with the plasmid containing survivin promoter sequence and stimulated with or without 10 ng/ml of HGF for 1 h. The promoter activity was analyzed in each well of the cultured medium using a Dual Glo™ luciferase assay system with a Turner Designs instrument (B). The measured luminescence of firefly luciferase was divided by renilla luciferase and the resulting quotient corresponded to the relative amounts of luciferase.
