Figure 8
From: Down-regulation of survivin suppresses uro-plasminogen activator through transcription factor JunB
Effects of survivin on cell proliferation and apoptosis. Cells (1 × 103/well) and stable survivin-shRNA cells were seeded in 96-well plates with DMEM media supplemented with 5% FBS and incubated for 24 h. After serum-starvation for 24 h, cell were treated with 10 ng/ml of HGF for 72 h. Cell proliferation was measured by MTT assays and expressed as a percentage of HGF-untreated control cells (A). Values are the means ± SD of three independent experiments. To see effects of survivin on HGF-mediated apoptosis, stable survivin-shRNA cells and control cells were treated with or without 10 ng/ml of HGF for 1 h and propidium iodide (PI). Fluorescence intensity of propidium iodide was analyzed by flow cytometry (B). Values are the means ± SD of three independent experiments.
